ENVIRONMENT

Located on the Health Sciences Campus (HSC) of USC, with satellite locations for interdisciplinary research staff on University Park Campus (UPC), the USC School of Pharmacy enjoys access to numerous classrooms, laboratories, core facilities and offices, enabling collaboration with colleagues across the USC research ecosystem, as well as nationally and internationally. USC School of Pharmacy faculty are internationally renowned for innovation in the design, discovery, targeting and delivery of novel therapies that improve human health—particularly in the treatment of neurodegenerative diseases, cancer, cardiovascular ailments, diabetes and immune system disorders. 
The School maintains a long tradition of interdisciplinary collaboration across academic departments, industry and government—conducting cutting-edge translational research that encompasses the full spectrum from basic laboratory discoveries to clinical care advances, and sparking real breakthroughs on complex and pervasive health challenges. As part of the University of Southern California, the USC School of Pharmacy offers a wide range of resources for scientists; the School maintains multiple core laboratories with state-of-the-art equipment suites and appropriate research staff support, supplementing facilities that are not typically maintained in individual labs. The School’s location on a major health sciences campus provides additional access to advanced facilities, and researchers throughout the University.

Facilities

The USC School of Pharmacy offers a wide range of cutting-edge resources for use by Faculty and Students. The School maintains three core laboratories with state-of-the-art equipment suites and appropriate research staff support, providing facilities not typically maintained in individual labs. 

The Translational Research Laboratory, on the Jane and Gale Bensussen Floor of the Pharmaceutical Sciences Center, promotes translational therapeutic discovery, with an emphasis on medium- and high-throughput technologies that are suited to the modern drug development endeavor. Capabilities of the lab include:

•    Fast gene expression profiling and genotyping using high-capacity real-time PCR systems
•    Automated fluorescence imaging capabilities for multiwell samples & multilabel samples including fluorescence measurement, time-resolution, polarization, luminescence and absorbance
•    Rapid analysis of cellular component dynamics by advanced spectrophotometry and high-content multiplex array systems
•    Simultaneous multiwell measurement of oxygen-dependent and -independent metabolism in cultured cells or isolated mitochondria
•    Optimization of fluorescence probes using scanning spectrophotometry.
•    Measurement of hydrodynamic particle radii, including polymers, soluble proteins and suspended particles.
•    Multiple instruments and techniques for gene transfer, expression, quantification and isolation.

The Histology Laboratory, provides full service histology preparative and embedding services, as well as all facilities necessary for individual researchers to create microscope slides from tissue samples. Tissues may be paraffin-embedded, sectioned or stained in the laboratory. Sectioning can involve making microtome, vibratome or cryostat thin slices. Color photographs of microscopic images can be produced for publication. Expert advice is available concerning staining techniques and the interpretation of microscopic images.

The Lentiviral Laboratory, in the Pharmaceutical Sciences Center, provides resources for the production of small-scale lentiviral stocks containing a given gene of interest and lentiviral titrations, concentrations and transductions of cells of interest.

Brinton Laboratory

The Brinton research environment consists of 2500 square feet of usable lab space divided into two laboratories, one for cellular and biochemical imaging and the other for biochemical and molecular biology analyses. Contiguous with the imaging laboratory is a 400 square ft isolated tissue culture facility equipped with 2 laminar flow hoods, four incubators, 2 refrigerators and microscopes. The imaging laboratory is equipped with a Zeiss based 3i Marianas™ digital microscopy workstation, an Olympus based BioQuant morphological imaging system and an iIntracellular calcium imaging system. The biochemical and molecular biology laboratory is equipped with instrumentation necessary to conduct biochemical signaling, genomic and proteomic analyses.  Contiguous of the Brinton research environment is the USC School of Pharmacy Translational Research Laboratory.

The Brinton research administration consists of two 150 sq. ft. offices adjacent to the Brinton laboratories, one for Dr. Brinton and one for her Project Manager, Claudia Lopez. 

Videoconferencing:  Brinton Lab office, administrative office, conference room and laboratory are equipped with iMac systems specifically configured for group videoconferencing for integration of projects across multiple performance sites. USC School of Pharmacy has a dedicated video conferencing room for large-scale meetings across institutions and working groups.